Authors
Azcuna, M. A.; Concepcion, G. P.; Zaulda, F.; Foronda, L.; De la Cruz, N.; Okino, T.; Anas, A.,
2011
Abstract
Four actinomycin compounds were isolated from an pactinomycte associated with a Petrosia (Strongylophora) sp. marine sponge. The bacterium was identified as Streptomyces sp. based on API and BIOLOG analysis. 16s rRNA gene sequence phylogeny identified the closest match as Streptomyces rubinigosohelvolus. The major compound was identified as Actinomycin D based on mass spectrometry, H-NMR, and C-NMR data. This compound showed biological activities against Staphylococcus aureus, Mycobacterium tuberculosis, and mammalian cells. This is the first report of Actinomycin D isolated from Streptomyces rubinigosohelvolus. Mass spectrometry and H-NMR analysis of the three minor compounds suggest they are actinomycin analogues, and further studies will be directed towards elucidating their structures and testing them for anti-bacterial and cytotoxic biological activities.